山羊 anti-Armenian Hamster IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 山羊, Golden Syrian Hamster, Armenian Hamster
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Armenian/Golden Syrian Hamster IgG and Armenian/Golden Syrian Hamster Serum.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Armenian and Golden Syrian Hamster IgG, whole molecule
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,000
Application Note: Anti Golden Syrian & Armenian Hamster IgG (H&L) antibody is suitable for Elisa, Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:10,000 - 1:50,000
Western Blot Dilution: 1:1,000 - 1:5,000
- 说明
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Anti-Golden Syrian & Armenian hamster IgG antibody is ideal for investigators involved in serum component protein research.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. - 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: goat anti-Golden Syrian & Armenian Hamster IgG biotin conjugated antibody, goat anti-Hamster IgG biotin conjugated antibody
Background: Anti-Golden Syrian & Armenian Hamster IgG Biotin Antibody generated in goat detects Golden Syrian & Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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