山羊 anti-小鼠 IgG, IgM Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
Rockland 610-105-b72
ELISA, WB, IHC
Polyclonal
IgG
Alkaline Phosphatase (AP)
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- 抗原 See all IgG, IgM products
- IgG, IgM
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Supplier Product No.
- 610-105-b72
- Supplier
- Rockland
- 原理
- Mouse IgG + IgM (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against to Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins.
- 产品特性
- Anti-Mouse IgG IgA IgM Alkaline Phosphatase Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM.
- 纯化方法
- This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
- Optional[Immunogen]: Mouse IgG and IgM whole molecules
- 亚型
- IgG
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Goat anti-Mouse IgG, IgM antibody (HRP) - Preadsorbed
ELISA, WB, IHC Polyclonal IgG HRP
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- 应用备注
- Application Note: Anti-Mouse IgG +IgM Alkaline Phosphatase conjugate has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:15,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.1 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 有效期
- 12 months
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- 抗原
- IgG, IgM
- 别名
- IgG,IgM (IgG, IgM 产品)
- 物质类
- Antibody
- 背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Alkaline Phosphate Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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