小鼠 anti-人 IgG (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 多克隆
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标记
- TRITC
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应用范围
- Fluorescence Microscopy (FM)
- 特异性
- Human IgG (H&L) Antibody Rhodamine ConjugatedAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG and Human Serum.
- 交叉反应
- 人
- 产品特性
- The antibody was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Human IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 2.1
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Goat anti-Human IgG (Fc Region) antibody (PE) - Preadsorbed
FACS, FM Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Human IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Goat anti-Human IgG (Fc Region) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Rabbit anti-Human IgG (Fc Region) antibody (FITC) - PreadsorbedFLISA, FM Polyclonal IgG FITC
Goat anti-Human IgG (Fc Region) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Human IgG (Fc Region) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Chicken anti-Human IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Rabbit anti-Human IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC Fab fragment
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- 应用备注
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Application Note: Mouse Anti-Human IgG Rhodamine conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
IF Microscopy Dilution: 1:500-1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
Centrifuge product if not completely clear after standing at room temperature. - 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: mouse anti-Human IgG rhodamine conjugated Antibody, mouse anti-Human IgG Antibody TRITC conjugation
Background: Anti-Human IgG (H&L) Rhodamine generated in mouse detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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