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山羊 anti-人 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Rockland 609-1316-0100 ELISA, IHC, WB Polyclonal IgG HRP
Rockland
产品编号 ABIN1043965
Supplier Product No.: 609-1316-0100
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2402
    • 511
    • 417
    • 148
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
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    • 1
    • 1
    Heavy & Light Chain
    适用
    • 719
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    宿主
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    • 11
    山羊
    克隆类型
    • 3389
    • 175
    • 1
    多克隆
    标记
    • 583
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    HRP
    应用范围
    • 2032
    • 1981
    • 1835
    • 1784
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    • 896
    • 486
    • 420
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    609-1316-0100
    Supplier
    Rockland
    原理
    Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
    交叉反应 (详细)
    Minimal crossreactivity against MOUSE Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum.  No reaction was observed against Mouse Serum Proteins.
    产品特性
    Anti-Human IgG (gamma chain) Peroxidase generated in goat detects human Immunoglobulin G (gamma chain).
    纯化方法
    Human IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    免疫原

    Immunogen: Human IgG whole molecule

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注
    Application Note: Anti-Human IgG (H&L) Peroxidase Conjugate has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:400,000 - 1:600,000 Other: User Optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • Muñoz, Beltrán-Alzate, Duthie, Serrano-Coll, Cardona-Castro: "Comparison of Enzyme-Linked Immunosorbent Assay Using Either Natural Octyl Disaccharide-Leprosy IDRI Diagnostic or Phenolic Glycolipid-I Antigens for the Detection of Leprosy Patients in Colombia." in: The American journal of tropical medicine and hygiene, Vol. 98, Issue 1, pp. 274-277, (2018) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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