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驴 anti-豚鼠 IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, WB Polyclonal IgG unconjugated
产品编号 ABIN1043951
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
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    Heavy & Light Chain
    适用
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    豚鼠
    宿主
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    克隆类型
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    多克隆
    应用范围
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    ELISA, Western Blotting (WB)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum.
    交叉反应
    豚鼠
    纯化方法
    Preadsorption: immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads
    过滤
    Sterile filtered
    免疫原

    Immunogen: Guinea Pig IgG whole molecule

    亚型
    IgG
  • 应用备注

    Application Note: Anti-Guinea Pig IgG antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:25,000 - 1:75,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: None

    储存液
    Without preservative
    注意事项
    Centrifuge product if not completely clear after standing at room temperature.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Donkey Anti-Guinea Pig IgG (H&L) Antibody Pre-Adsorbed, Donkey Anti Guinea Pig IgG

    Background: Anti-Guinea Pig IgG Antibody generated in donkey detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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