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驴 anti-小鸡 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Rockland 603-703-c37 ELISA, IHC, WB Polyclonal IgG HRP
Rockland
产品编号 ABIN1043931
Supplier Product No.: 603-703-c37
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
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    Heavy & Light Chain
    适用
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    小鸡
    宿主
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    克隆类型
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    多克隆
    标记
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    HRP
    应用范围
    • 2032
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    603-703-c37
    Supplier
    Rockland
    原理
    Chicken IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
    交叉反应 (详细)
    Minimal crossreactivity against Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Donkey Serum, Chicken IgG and Chicken Serum.   No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
    产品特性
    Anti-Chicken IgG Fluorescein Antibody generated in donkey detects chicken IgY.
    纯化方法
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation and extensive dialysis against the buffer stated above.
    免疫原
    Optional[Immunogen]: Chicken IgG whole molecule
    亚型
    IgG
  • 应用备注
    Application Note: Anti-Chicken IgG (H&L) Peroxidase has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:65,000
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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