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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 小鸡
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宿主
- 驴
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Chicken IgG and Chicken Serum.
- 交叉反应
- 小鸡
- 交叉反应 （详细）
- Pre-Adsorbed
- 产品特性
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Chicken IgY antibody generated in donkey detects specifically chicken IgG. This anti-Chicken secondary antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
- 纯化方法
- Anti-Chicken IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Chicken IgG whole molecule
- 亚型
- IgG
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Goat anti-Chicken IgG (Heavy & Light Chain) antibody (HRP)
ELISA, WB Polyclonal IgG HRP
Rabbit anti-Chicken IgG (F(ab')2 Region) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Chicken IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Chicken IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Chicken IgG (Heavy & Light Chain) antibodyELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Goat anti-Chicken IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Chicken IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Chicken IgG (Heavy & Light Chain) antibody (Biotin)ELISA, IHC, WB Polyclonal IgG Biotin
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Chicken IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.12 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None
- 储存液
- Without preservative
- 注意事项
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Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
Dilute only prior to immediate use. - 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Donkey anti-Chicken IgG Antibody, Donkey anti-Chicken IgG Pre-adsorbed Antibody
Background: Anti-Chicken IgG Antibody generated in donkey detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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