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兔 anti-大鼠 IgM Antibody

Rockland 612-4107 ELISA, IHC, WB Polyclonal IgG unconjugated
Rockland
产品编号 ABIN102711
Supplier Product No.: 612-4107
发货至: 中国
  • 抗原 See all IgM products
    IgM
    适用
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    克隆类型
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    多克隆
    应用范围
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    612-4107
    Supplier
    Rockland
    原理
    Rat IgM (mu chain) Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgM and Rat Serum.  No reaction was observed against other rat heavy or light chain proteins.
    产品特性
    Anti-Rat IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the rat IgG molecule. Rat IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind rat IgG F(c) will not bind rat IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
    纯化方法
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose followed by solid phase adsorption(s).
    过滤
    Sterile filtered
    免疫原
    Optional[Immunogen]: Rat IgM whole molecule
    亚型
    IgG
  • 应用备注
    Application Note: Anti-Rat IgM mu heavy chain antibody is tested by western blot and suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:5,000 - 1:25,000 ELISA Dilution: 1:50,000 - 1:250,000 Other: User Optimized
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    2.4 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
    有效期
    12 months
  • 抗原
    IgM
    Abstract
    IgM 产品
    物质类
    Antibody
    背景
    Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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