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- 抗原 See all IgM products
- IgM
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 612-1107
- Supplier
- Rockland
- 原理
- Rat IgM (mu chain) Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
- 产品特性
- Rat IgA antibodies detects the alpha chain of the rat IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes. Anti-Rat IgA antibody is ideal for investigators involved in serum component research.
- 纯化方法
- Anti-RAT IgM (mu chain) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
- Optional[Immunogen]: Rat IgM whole molecule
- 亚型
- IgG
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Goat anti-Rat IgM (Heavy Chain) antibody
ELISA, FACS, IHC, WB Polyclonal IgG unconjugated
Goat anti-Rat IgM (Heavy Chain) antibody (Texas Red (TR))ELISA, FACS, IHC Polyclonal IgG Texas Red (TR)
Goat anti-Rat IgM (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
Goat anti-Rat IgM (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Rat IgM (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rat IgM (Heavy Chain) antibody (Biotin)ELISA, FACS, IHC, WB Polyclonal IgG Biotin
Goat anti-Rat IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Mouse anti-Rat IgM antibodyELISA, FACS Monoclonal M2A1 IgG1 unconjugated
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- 应用备注
- Application Note: Anti-RAT IgM (mu chain) (GOAT) Antibody has been tested by ELISA and western blot and is assayed against Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Rat IgM antibody is suitable for immunoassays including western blot and ELISA. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:1,000 to 1:10,000 ELISA Dilution: 1:80,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.16 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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