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山羊 anti-兔 IgM (Heavy Chain) Antibody

ELISA, IHC, WB Polyclonal unconjugated
产品编号 ABIN102693
发货至: 中国
  • 抗原 See all IgM products
    IgM
    抗原表位
    • 162
    • 122
    • 87
    • 57
    • 26
    • 9
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    Heavy Chain
    适用
    • 225
    • 156
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    宿主
    • 364
    • 231
    • 85
    • 18
    • 14
    • 5
    • 4
    • 1
    • 1
    山羊
    克隆类型
    • 533
    • 88
    多克隆
    应用范围
    • 374
    • 281
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    • 220
    • 130
    • 124
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgM µ chain
    产品特性
    Concentration Definition: by Refractometry
    纯化方法
    This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Rabbit IgM and Rabbit Serum.  No reaction was observed against Rabbit IgG.
    免疫原

    Immunogen: Rabbit IgM mu heavy chain

  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Rabbit IgM antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    说明

    Protein Concentration: 90.0 mg/mL (by Refractometry)

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    90 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgM
    Abstract
    IgM 产品
    物质类
    Antibody
    背景

    Synonyms: goat anti-Rabbit IgM (mu) Antibody, goat anti Rabbit IgM mu

    Background: Anti-Rabbit IgM antibody generated in goat specifically detects rabbit IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rabbit IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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