山羊 anti-小鼠 IgM Antibody (HRP)
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- 抗原 See all IgM products
- IgM
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 610-1307
- Supplier
- Rockland
- 原理
- Mouse IgM (mu chain) Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against other mouse heavy or light chain proteins.
- 产品特性
- MOUSE IgG F(ab')2 (GOAT) Antibody is generated in goat detects specifically Mouse IgG F(ab')2 fragment. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Mouse IgG F(ab')2 antibody is ideal for investigators involved in serum protein component research.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
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Immunogen: Mouse IgM whole molecule
Immunogen Type: Native Protein
- 亚型
- IgG
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Goat anti-Mouse IgM (Heavy Chain) antibody (TRITC)
ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Mouse IgM (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
Goat anti-Mouse IgM (Heavy Chain) antibody (Biotin)ELISA, FACS, IHC, WB Polyclonal IgG Biotin
Goat anti-Mouse IgM (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgM antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
Goat anti-Mouse IgM antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Goat anti-Mouse IgM (Heavy Chain) antibodyELISA, FACS, IHC, WB Polyclonal IgG unconjugated
Goat anti-Mouse IgM (Heavy Chain) antibody (beta-Gal)ELISA, IHC Polyclonal IgG beta-Gal
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- 应用备注
- Application Note: Mouse IgM (mu chain) peroxidase conjugated Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:2,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:30,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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