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山羊 anti-小鼠 IgM (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
产品编号 ABIN102676
发货至: 中国
  • 抗原 See all IgM products
    IgM
    抗原表位
    • 162
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    • 87
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    • 26
    • 10
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    • 1
    Heavy & Light Chain
    适用
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    小鼠
    宿主
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    山羊
    克隆类型
    • 533
    • 88
    多克隆
    标记
    • 121
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    Biotin
    应用范围
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgM µ chain
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgM whole molecule

    亚型
    IgG
    Labeling Ratio
    10-20
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Mouse IgM (mu chain) biotin conjugated Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

    ELISA Dilution: 1:10,000 - 1:50,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    2.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Aliquot to Avoid repeated freezing and thawing.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgM
    Abstract
    IgM 产品
    物质类
    Antibody
    背景

    Synonyms: Goat Anti-Mouse IgM (mu chain) Antibody Biotin Conjugated, Goat Anti Mouse IgM mu chain Biotin Conjugated Antibody

    Background: Anti-Mouse IgM (mu chain) biotin conjugated antibody generated in goat detects specifically mouse IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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