山羊 anti-小鼠 IgM (Heavy & Light Chain) Antibody - Preadsorbed
-
- 抗原 See all IgM products
- IgM
-
抗原表位
- Heavy & Light Chain
-
适用
- 小鼠
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgM µ chain
- 交叉反应
- 小鼠
- 产品特性
-
Anti-Mouse IgM (mu chain) antibody generated in goat detects specifically mouse IgM (mu chain). This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm - 纯化方法
- Preadsorption: Solid phase absorption
- 过滤
- Sterile filtered
- 免疫原
-
Immunogen: Mouse IgM whole molecule
Immunogen Type: Native Protein
- 亚型
- IgG
-
-
- 应用备注
-
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Mouse IgM (mu chain) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.
ELISA Dilution: 1:20,400 - 1:40,400
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 2.1 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
-
- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
-
Synonyms: goat anti-Mouse IgM (mu chain) Antibody, Gt-a-Mouse (mu chain) antibody, Mouse IgM (mu chain) Antibody in goat, Goat anti-mouse IgM Secondary Antibody
Background: Anti-Mouse IgM (mu chain) antibody generated in goat detects specifically mouse IgM (mu chain). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
-