山羊 anti-猴 IgM (Chain mu) Antibody (Biotin) - Preadsorbed
(1 reference)-
- 抗原 See all IgM products
- IgM
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抗原表位
- Chain mu
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适用
- 猴
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgM µ chain
- 交叉反应
- 猴
- 产品特性
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Anti-Monkey IgM (mu chain) biotin conjugated antibody generated in goat detects specifically monkey IgM (mu chain). This secondary biotin conjugated antibody anti-Monkey is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm - 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Monkey IgM whole molecule
- 亚型
- IgM
- Labeling Ratio
- 10-20
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Goat anti-Monkey IgM (Chain mu) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgM unconjugated
Goat anti-Monkey IgM (Chain mu) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgM HRP
Goat anti-Monkey IgM (Chain mu) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Monkey IgG (mu chain) Biotin conjugated Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:20,000 - 1:300,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Aliquot to Avoid repeated freezing and thawing.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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: "Analysis of Complement-Mediated Lysis of Simian Immunodeficiency Virus (SIV) and SIV-Infected Cells Reveals Sex Differences in Vaccine-Induced Immune Responses in Rhesus Macaques." in: Journal of virology, Vol. 92, Issue 19, (2018) (PubMed).
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: "Analysis of Complement-Mediated Lysis of Simian Immunodeficiency Virus (SIV) and SIV-Infected Cells Reveals Sex Differences in Vaccine-Induced Immune Responses in Rhesus Macaques." in: Journal of virology, Vol. 92, Issue 19, (2018) (PubMed).
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat Anti-Monkey IgM Antibody biotin Conjugation, Goat Anti-Monkey IgM biotin Conjugated Antibody
Background: Anti-Monkey IgM (mu chain) biotin conjugated antibody generated in goat detects specifically monkey IgM (mu chain). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Monkey IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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