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山羊 anti-人 IgM Antibody (HRP)

Name: 山羊 anti-人 IgM Antibody (HRP)
SKU: ABIN102628
Availability: OutOfStock

Rockland 609-1307 ELISA, IHC, WB Polyclonal IgG HRP

ELISA

1 image

(3 references)

产品编号 ABIN102628

Supplier Product No.: 609-1307

发货至: 中国

Datasheet as PDF

抗原 See all IgM products

IgM
适用
223

159

91

42

34

34

29

24

17

14

13

13

13

13

12

4

2
人
宿主
372

231

84

19

14

5

4

1

1
山羊
克隆类型
539

90
多克隆
标记
121

86

85

66

34

32

26

24

14

12

11

11

11

11

11

11

11

10

9

9

9

7

7

6

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

2

2

2

2

2

2

2

2

1
HRP
应用范围
383

288

282

242

182

137

91

91

64

62

54

53

45

30

18

17

10

6

5

3

3

3

2

2

1

1

1

1

1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.

609-1307

Supplier

Rockland

原理

Human IgM (mu chain) Antibody Peroxidase Conjugated

交叉反应（详细）

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against other human heavy or light chain proteins.

产品特性

Anti-Human IgA Peroxidase Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

纯化方法

Human IgM (mu chain) Antibody, Peroxidase Conjugated, was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Optional[Immunogen]: Human IgM whole molecule

亚型

IgG
Mouse anti-Human IgM antibody (HRP)
ELISA, WB Monoclonal CH2 IgG1 HRP

Goat anti-Human IgM antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Human IgM antibody (FITC)
ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment

Goat anti-Human IgM (Heavy Chain) antibody (TRITC)
ELISA, FACS, IHC Polyclonal IgG TRITC

Goat anti-Human IgM (Heavy Chain) antibody (FITC)
ELISA, FACS, IHC Polyclonal IgG FITC

Goat anti-Human IgM (Heavy Chain) antibody (Texas Red (TR))
ELISA, FACS, IHC Polyclonal IgG Texas Red (TR)

Goat anti-Human IgM antibody (HRP) - Preadsorbed
ELISA, FLISA Polyclonal IgG HRP

Goat anti-Human IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Human IgM antibody (HRP)
ELISA, FACS, FLISA Polyclonal IgG HRP F(ab')2 fragment

Mouse anti-Human IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB Monoclonal SA-DA4 IgG1 Alkaline Phosphatase (AP)

应用备注

Application Note: Anti-Human IgM (mu heavy chain) Peroxidase has been tested by ELISA and is suitable for western blot, immunohistochemistry, and immunoassays where specificity to the immunoglobulin mu chain region is desired. Optimal concentrations in immunoassays should be determined by the researcher. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:180,000 Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

储存液

Gentamicin sulfate

注意事项

This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Crawford, Tempel, Streblow, Kreklywich, Smith, Picker, Nelson, Caposio: "Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice." in: Scientific reports, Vol. 7, Issue 1, pp. 937, (2018) (PubMed).

Yang, Jacobs, McCallen, Moore, Huckaby, Edelstein, Lai: "Analysis of Pre-existing IgG and IgM Antibodies against Polyethylene Glycol (PEG) in the General Population." in: Analytical chemistry, Vol. 88, Issue 23, pp. 11804-11812, (2018) (PubMed).

Zupancic, Blejec, Herman, Veber, Verbovsek, Korsic, Knezevic, Rozman, Turnsek, Gruden, Motaln: "Identification of plasma biomarker candidates in glioblastoma using an antibody-array-based proteomic approach." in: Radiology and oncology, Vol. 48, Issue 3, pp. 257-66, (2014) (PubMed).
抗原

IgM

Abstract

IgM 产品

物质类

Antibody

背景

Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.