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- 抗原 See all IgM products
- IgM
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适用
- 小鸡
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 603-103-007
- Supplier
- Rockland
- 原理
- Chicken IgM (mu chain) Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Chicken IgM and Chicken Serum. No reaction was observed against other chicken heavy or light chain proteins.
- 产品特性
- Anti-Chicken IgG Peroxidase Antibody generated in goat detects chicken IgY.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
- Optional[Immunogen]: Chicken IgM whole molecule
- 亚型
- IgG
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Mouse anti-Chicken IgM antibody (PE)
ELISA, FACS Monoclonal M-1 IgG2b PE
Mouse anti-Chicken IgM antibody (SPRD)ELISA, FACS Monoclonal M-1 IgG2b SPRD
Mouse anti-Chicken IgM antibody (Biotin)ELISA, FACS Monoclonal M-1 IgG2b Biotin
Mouse anti-Chicken IgM antibody (APC)ELISA, FACS, FLISA Monoclonal M-1 IgG2b APC
Mouse anti-Chicken IgM antibody (FITC)ELISA, FACS Monoclonal M-1 IgG2b FITC
Goat anti-Chicken IgM antibodyELISA, IHC, WB Polyclonal IgG unconjugated
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- 应用备注
- Application Note: Anti-Chicken IgM Peroxidase Conjugated antibody has been tested by ELISA and western blot. This product has been assayed against 1.0 μg of Chicken IgM in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 is suggested for this product. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Chicken IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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