山羊 anti-小鸡 IgM Antibody (Alkaline Phosphatase (AP))
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- 抗原 See all IgM products
- IgM
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适用
- 小鸡
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 603-105-007
- Supplier
- Rockland
- 原理
- Chicken IgM (mu chain) Antibody Alkaline Phosphatase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Chicken IgM and Chicken Serum. No reaction was observed against other chicken heavy or light chain proteins.
- 产品特性
- Anti-Chicken IgG Alkaline Phosphatase Antibody generated in goat detects chicken IgY.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
- Optional[Immunogen]: Chicken IgM whole molecule
- 亚型
- IgG
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Mouse anti-Chicken IgM antibody (PE)
ELISA, FACS Monoclonal M-1 IgG2b PE
Mouse anti-Chicken IgM antibody (SPRD)ELISA, FACS Monoclonal M-1 IgG2b SPRD
Mouse anti-Chicken IgM antibody (Biotin)ELISA, FACS Monoclonal M-1 IgG2b Biotin
Mouse anti-Chicken IgM antibody (APC)ELISA, FACS, FLISA Monoclonal M-1 IgG2b APC
Mouse anti-Chicken IgM antibody (FITC)ELISA, FACS Monoclonal M-1 IgG2b FITC
Goat anti-Chicken IgM antibodyELISA, IHC, WB Polyclonal IgG unconjugated
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- 应用备注
- Application Note: Anti-Chicken IgM Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:200 - 1:2,000 ELISA Dilution: 1:8,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Chicken IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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