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兔 anti-小鼠 IgG2b Antibody (HRP)

Rockland 610-4342 ELISA, IHC, WB Polyclonal IgG HRP

Characterization of antibody responses induced by DNA vaccines expressing particulate and non-particulate core antigens. (兔 anti-小鼠 IgG2b Antibody (HRP))

ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation tested against purified Mouse IgG2b HRP. (兔 anti-小鼠 IgG2b Antibody (HRP))

Induction of HBV core-specific antibodies in mice. (兔 anti-小鼠 IgG2b Antibody (HRP))

Induction of HBV core-specific antibodies in B6 and TLR7-/- mice. (兔 anti-小鼠 IgG2b Antibody (HRP))

Expression and characterization of a mutant HBV-stCAAA and HBV-stCAAAY132A antigen. (兔 anti-小鼠 IgG2b Antibody (HRP))

Characterization of antibody responses induced by DNA vaccines expressing particulate and non-particulate core antigens. (兔 anti-小鼠 IgG2b Antibody (HRP))

Characterization of antibody responses induced by DNA vaccines expressing particulate and non-particulate core antigens. (兔 anti-小鼠 IgG2b Antibody (HRP))

7 images

(1 reference)

产品编号 ABIN102401

Supplier Product No.: 610-4342

发货至: 中国

Datasheet as PDF

抗原 See all IgG2b products

IgG2b
适用
88

23
小鼠
宿主
59

31

12

8

1
兔
克隆类型
70

20
多克隆
标记
20

11

11

8

8

5

5

5

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
HRP
应用范围
54

47

35

30

29

21

14

9

7

5

5

5

4

3

3

2

1

1

1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.

610-4342

Supplier

Rockland

原理

Mouse IgG2b (Gamma 2b chain) Antibody Peroxidase Conjugated

交叉反应（详细）

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse or human heavy or light chain isotypes.

产品特性

Anti-Mouse IgG2a heavy chain antibody generated in rabbit detects specifically Mouse IgG2a heavy chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

纯化方法

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Immunogen: Anti-Mouse IgG2b was produced by repeated immunization with mouse IgG2b heavy chain in rabbit.
Immunogen Type: Native Protein

亚型

IgG
Goat anti-Mouse IgG2b (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Mouse IgG2b (Heavy Chain) antibody (FITC)
ELISA, IHC, FACS Polyclonal IgG FITC

Goat anti-Mouse IgG2b (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC

Goat anti-Mouse IgG2b antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE

Goat anti-Mouse IgG2b antibody (Alkaline Phosphatase (AP))
ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Mouse IgG2b antibody
ELISA, FLISA Polyclonal IgG unconjugated

Goat anti-Mouse IgG2b (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

Rat anti-Mouse IgG2b antibody
ELISA, FACS, FLISA Monoclonal SB74g IgG2b unconjugated

Goat anti-Mouse IgG2b antibody (Biotin)
ELISA, FLISA Polyclonal IgG Biotin

Rat anti-Mouse IgG2b antibody (FITC)
ELISA, FACS, FLISA Monoclonal SB74g IgG2b FITC

应用备注

Application Note: Anti-Mouse IgG2b peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:65,000 Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

储存液

Gentamicin sulfate

注意事项

This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Krieger, Stifter, Riedl, Schirmbeck: "Cationic domains in particle-forming and assembly-deficient HBV core antigens capture mammalian RNA that stimulates Th1-biased antibody responses by DNA vaccination." in: Scientific reports, Vol. 8, Issue 1, pp. 14660, (2018) (PubMed).
抗原

IgG2b

Abstract

IgG2b 产品

物质类

Antibody

背景

Anti-Mouse IgG2b peroxidase conjugated antibody generated in rabbit detects specifically mouse IgG2b. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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