兔 anti-小鼠 IgG2a (Heavy Chain) Antibody (FITC) - Preadsorbed
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- 抗原 See all IgG2a products
- IgG2a
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抗原表位
- Heavy Chain
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 特异性
- IgG2a
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads
- 免疫原
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Immunogen: Mouse IgG2a heavy chain
- 亚型
- IgG
- Labeling Ratio
- 3.7
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Rat anti-Mouse IgG2a antibody
ELISA, FACS Monoclonal SB84a IgG1 unconjugated
Goat anti-Mouse IgG2a (Heavy Chain) antibody (FITC) - PreadsorbedFACS, IHC, WB, FLISA, FM Polyclonal IgG FITC
Rat anti-Mouse IgG2a antibody (PE)ELISA, FACS Monoclonal SB84a IgG1 PE
Goat anti-Mouse IgG2a (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Mouse IgG2a antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Goat anti-Mouse IgG2a antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
Goat anti-Mouse IgG2a antibody (FITC)ELISA, FLISA Polyclonal IgG FITC
Goat anti-Mouse IgG2a antibody (PE-Cy7) - PreadsorbedELISA, FACS, FLISA Polyclonal IgG PE-Cy7
Goat anti-Mouse IgG2a antibody (Alkaline Phosphatase (AP))ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG2a antibodyELISA, FLISA Polyclonal IgG unconjugated
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- 应用备注
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 说明
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Excitation/Emission wavelength: 494 nm/514 nm
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG2a
- Abstract
- IgG2a 产品
- 物质类
- Antibody
- 背景
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Synonyms: Rabbit Anti-Mouse IgG2a (gamma 2a chain) Antibody fluorescein Conjugated, Rabbit Anti-Mouse IgG2a Antibody FITC Conjugation
Background: Anti-Mouse IgG2a Fluorescein Antibody generated in rabbit detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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