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兔 anti-小鼠 IgG1 Antibody (Texas Red (TR))

Name: 兔 anti-小鼠 IgG1 Antibody (Texas Red (TR))
SKU: ABIN102341
Availability: OutOfStock

Rockland 610-4940 WB, FACS, FLISA, DB, FM Polyclonal IgG Texas Red (TR)

Western blot of Texas conjugated Rabbit Anti-Mouse IgG1 (Gamma 1 chain) secondary antibody. (兔 anti-小鼠 IgG1 Antibody (Texas Red (TR)))

1 image

(1 reference)

产品编号 ABIN102341

Supplier Product No.: 610-4940

发货至: 中国

Datasheet as PDF

抗原 See all IgG1 products

IgG1
适用
94

24

23

9

7

5

4

4

3

2

1
小鼠
宿主
61

41

39

15

10

6

1
兔
克隆类型
92

60
多克隆
标记
46

21

21

18

11

8

7

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Texas Red (TR)
应用范围
90

59

50

46

29

19

18

16

13

11

10

9

8

6

4

3

2

2

2
Western Blotting (WB), Flow Cytometry (FACS), FLISA, Dot Blot (DB), Fluorescence Microscopy (FM)

Supplier Product No.

610-4940

Supplier

Rockland

原理

Mouse IgG1 (Gamma 1 chain) Antibody Texas Red™ Conjugated

交叉反应（详细）

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse heavy or light chain isotypes.

产品特性

Anti-Mouse IgG F(ab')2 Texas Red Antibody generated in rabbit recognizes the dimeric Fab portion of the mouse IgG molecule. Mouse IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind mouse IgG F(c) will not bind mouse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

纯化方法

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Optional[Immunogen]: Mouse IgG1 heavy chain

亚型

IgG
Goat anti-Mouse IgG1 (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)

Rat anti-Mouse IgG1 antibody
ELISA, FACS Monoclonal SB77e IgG2b unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody
ELISA, WB, IHC, FACS Polyclonal IgG unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)
ELISA, IHC, FACS Polyclonal IgG FITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)
ELISA, WB, IHC, FACS Polyclonal IgG Biotin

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Cy5)
IHC, FACS Polyclonal IgG Cy5

Goat anti-Mouse IgG1 antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Goat anti-Mouse IgG1 antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE

应用备注

Application Note: Anti-Mouse IgG1 Texas Red Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
de Villiers, Houreld, Abrahamse: "Influence of Low Intensity Laser Irradiation on Isolated Human Adipose Derived Stem Cells Over 72 Hours and Their Differentiation Potential into Smooth Muscle Cells Using Retinoic Acid." in: Stem cell reviews, (2011) (PubMed).
抗原

IgG1

Abstract

IgG1 产品

物质类

Antibody

背景

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.