兔 anti-小鼠 IgG1 (Heavy Chain) Antibody (Alkaline Phosphatase (AP))
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- 抗原 See all IgG1 products
- IgG1
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抗原表位
- Heavy Chain
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- IgG1
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Mouse IgG1 heavy chain
- 亚型
- IgG
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Goat anti-Mouse IgG1 (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Mouse IgG1 (Heavy Chain) antibody - PreadsorbedELISA, WB, IHC Polyclonal IgG unconjugated
Rat anti-Mouse IgG1 antibodyELISA, FACS Monoclonal SB77e IgG2b unconjugated
Rat anti-Mouse IgG1 antibody (HRP)ELISA Monoclonal SB77E IgG2b HRP
Goat anti-Mouse IgG1 (Heavy Chain) antibodyELISA, WB, IHC, FACS Polyclonal IgG unconjugated
Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)ELISA, WB, IHC, FACS Polyclonal IgG Biotin
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Atto 647N) - PreadsorbedWB, FACS, FLISA, FM Polyclonal IgG Atto 647N
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Mouse IgG1 secondary antibody conjugated to Alkaline Phosphatase is available in a variety of formats. Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
ELISA Dilution: 1:5,000
Western Blot Dilution: 1:500 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原
- IgG1
- Abstract
- IgG1 产品
- 物质类
- Antibody
- 背景
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Synonyms: Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody alkaline phosphatase Conjugation, Rabbit Anti-Mouse IgG1 alk phos Conjugated Antibody
Background: Anti-Mouse IgG1 Alkaline Phosphatase Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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