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兔 anti-绵羊 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Rockland 613-4328 ELISA, IHC, WB Polyclonal IgG HRP
Rockland
产品编号 ABIN102262
Supplier Product No.: 613-4328
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
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    Heavy & Light Chain
    适用
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    绵羊
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    克隆类型
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    多克隆
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    HRP
    应用范围
    • 2032
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    613-4328
    Supplier
    Rockland
    原理
    Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
    交叉反应 (详细)
    Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG and Sheep Serum.  No reaction was observed against Human Serum Proteins.
    产品特性
    Anti-Sheep IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the sheep IgG molecule. Sheep IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind sheep IgG F(c) will not bind sheep IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG F(ab')2 is conjugated to Peroxidase.
    纯化方法
    Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    免疫原
    Optional[Immunogen]: Sheep IgG whole molecule
    亚型
    IgG
  • 应用备注
    Application Note: Anti-Sheep IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Immunohistochemistry Dilution: 1:300 - 1:1,500 Western Blot Dilution: 1:500 - 1:3,000 ELISA Dilution: 1:5,000 - 1:22,000 Other: User Optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
    有效期
    12 months
  • Bissoon-Haqqani, Moyana, Jonker, Maroun, Birnboim: "Nuclear expression of thymidylate synthase in colorectal cancer cell lines and clinical samples." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 54, Issue 1, pp. 19-29, (2006) (PubMed).

    Cripe, Gelfanov, Smith, Spigel, Phillips, Gabig, Jung, Fyffe, Hartman, Kneebone, Mercola, Burgess, Boswell: "Role for c-jun N-terminal kinase in treatment-refractory acute myeloid leukemia (AML): signaling to multidrug-efflux and hyperproliferation." in: Leukemia, Vol. 16, Issue 5, pp. 799-812, (2002) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    HRP Conjugated Secondary Antibody is designed for western blotting, ELISA and Immunohistochemistry. Horseradish peroxidase conjugated antibodies are ideal for antibody detection assays that require lot-to-lot consistency.
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