兔 anti-绵羊 IgG (Heavy & Light Chain) Antibody (TRITC)
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- TRITC
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应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)
- 原理
- Sheep IgG (H&L) Antibody Rhodamine Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum.
- 产品特性
- Anti-Rat IgG (H&L) Texas Red™ Antibody generated in chicken detects reactivity to Rat IgG.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
- Optional[Immunogen]: Sheep IgG whole molecule
- 亚型
- IgG
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Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (Biotin) - Preadsorbed
ELISA, IHC, WB, DB Polyclonal IgG Biotin
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (FITC)FACS, FLISA, FM, DB Polyclonal IgG FITC
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (Texas Red (TR))WB, FACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (FITC)WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (TRITC)WB, FACS, FLISA, FM Polyclonal IgG TRITC Fab fragment
Rabbit anti-Sheep IgG (F(ab')2 Region) antibody (FITC)FACS, FLISA, FM, DB Polyclonal IgG FITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedWB, FACS, FLISA, FM Polyclonal IgG FITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (TRITC)FM, DB Polyclonal IgG TRITC F(ab')2 fragment
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
- Application Note: Anti-Sheep IgG (H&L) Rhodamine Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG Antibody is conjugated to Rhodamine.
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