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兔 anti-绵羊 IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB, LF Polyclonal IgG unconjugated
产品编号 ABIN102249
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
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    Heavy & Light Chain
    适用
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    绵羊
    宿主
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    克隆类型
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    多克隆
    应用范围
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Lateral Flow (LF)
    特异性
    IgG (H&L)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: immunoaffinity chromatography using Sheep IgG coupled to agarose
    过滤
    Sterile filtered
    免疫原

    Immunogen: Sheep IgG whole molecule

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Sheep IgG antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

    ELISA Dilution: 1:50,000 - 1:200,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    2.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Rabbit Anti-Sheep IgG Antibody

    Background: Anti-Sheep IgG (H&L) generated in rabbit detects sheep Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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