兔 anti-大鼠 IgG (Heavy & Light Chain) Antibody - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG (H&L)
- 交叉反应
- 大鼠
- 产品特性
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Anti-Rat IgG (H&L) antibody generated in rabbit detects specifically rat IgG (H&L). This secondary antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm - 纯化方法
- Preadsorption: Solid phase absorption
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-Rat IgG (H&L) was produced by repeated immunization with rat IgG whole molecule in rabbit
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000-1:5,000
Application Note: Antibody anti-Rat has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. Antibody anti-Rat has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20,000 - 1:300,000
Western Blot Dilution: 1:2,000-1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.2 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: rabbit anti-Rat IgG (H&L) Antibody, Rabbit-a-Rat IgG (H&L), Rat IgG Antibody in rabbit, anti-Rat IgG Secondary Antibody
Background: Anti-Rat IgG (H&L) antibody generated in rabbit detects specifically rat IgG (H&L). This secondary antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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