兔 anti-大鼠 IgG (Heavy & Light Chain) Antibody
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG (H&L)
- 产品特性
- Concentration Definition: by Refractometry
- 纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Rat IgG and Rat Serum.
- 免疫原
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Immunogen: Rat IgG whole molecule
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Rat IgG antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 80 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: rabbit anti-Rat IgG Antibody, rabbit anti Rat IgG
Background: Anti-Rat IgG (H&L) generated in rabbit detects rat Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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