山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG (H&L)
- 产品特性
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Anti-RAT IgG (H&L) (GOAT) Antibody generated in sheep detects specifically rat IgG whole molecule. Anti-rat IgG antibody is ideal for investigators involved in serum protein component research.
Concentration Definition: by UV absorbance at 280 nm - 纯化方法
- Preadsorption: immunoaffinity chromatography using Rat IgG coupled to agarose beads
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Rat IgG whole molecule
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000-1:5,000
Application Note: Anti-RAT IgG (H&L) (GOAT) Antibody is suitable for ELISA, Western Blot and Immunohistochemistry applications. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:20,000-1:100,000
Western Blot Dilution: 1:2,000-1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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Motor neuron intrinsic and extrinsic mechanisms contribute to the pathogenesis of FUS-associated amyotrophic lateral sclerosis." in: Acta neuropathologica, Vol. 133, Issue 6, pp. 887-906, (2017) (PubMed).
: "Lacidipine attenuates apoptosis via a caspase-3 dependent pathway in human kidney cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 32, Issue 4, pp. 1040-9, (2014) (PubMed).
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Motor neuron intrinsic and extrinsic mechanisms contribute to the pathogenesis of FUS-associated amyotrophic lateral sclerosis." in: Acta neuropathologica, Vol. 133, Issue 6, pp. 887-906, (2017) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat anti-rat IgG, Rat antibody, rat antibodies, immunoglobulin
Background: Anti-RAT IgG (H&L) (GOAT) Antibody generated in goat detects specifically rat IgG whole molecule. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-rat IgG antibody is ideal for investigators involved in serum protein component research.
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