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山羊 anti-兔 IgG (Fc Region) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
产品编号 ABIN102079
发货至: 中国
  • 抗原 See all IgG products
    IgG
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    克隆类型
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    多克隆
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    交叉反应
    产品特性
    Anti-Rabbit IgG F(c) antibody generated in goat detects specifically rabbit IgG F(c). This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Synonyms: Rabbit Antibody F(c), Gt-a-Rabbit IgG F(c), Rabbit Antibody in Goat, Rabbit F(c) Secondary Antibody
    纯化方法
    Preadsorption: Solid phase absorption
    过滤
    Sterile filtered
    免疫原

    Immunogen: Anti Rabbit IgG F(c) was produced by repeated immunization with rabbit IgG F(c) fragment in goat.

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Rabbit IgG F(c) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    说明

    Concentration (detection method): 2.0 mg/mL (by UV absorbance at 280 nm)

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    2.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: goat anti-Rabbit F(c) Antibody, Gt-a-Rabbit IgG F(c), Rabbit Antibody in Goat, Goat anti-Rabbit F(c) fragment Secondary Antibody

    Background: Anti-Rabbit IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

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