山羊 anti-兔 IgG (Heavy & Light Chain) Antibody
(3 references)
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Fluorescence Microscopy (FM), Immunoprecipitation (IP), Dot Blot (DB)
- Supplier Product No.
- 611-1102
- Supplier
- Rockland
- 原理
- Rabbit IgG (H&L) Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
- 产品特性
- Anti-Rabbit IgG Antibody Texas Red™ generated in goat detects rabbit IgG.
- 纯化方法
- Rabbit Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.
Immunogen Type: Native Protein
- 亚型
- IgG
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Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, CUT&RUN, CUT&Tag Polyclonal IgG unconjugated
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC)WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)High-quality product from Rockland ELISA, IHC, WB, DB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 800)ELISA, WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Rabbit IgG antibody (DyLight 488)WB, IF, FLISA, FM, DB, MA Polyclonal IgG DyLight 488
Goat anti-Rabbit IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
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- 应用备注
- Application Note: Anti-Rabbit IgG (H&L) has been tested by ELISA, dot blot, and western blot. This antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:10,000 - 1:60,000 IF Microscopy Dilution: User Optimized Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.2 mg/mL
- 缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Preservative:0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store Rabbit Secondary Antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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: "Sesquiterpene lactone 6-O-angeloylplenolin reverses vincristine resistance by inhibiting YB-1 nuclear translocation in colon carcinoma cells." in: Oncology letters, Vol. 15, Issue 6, pp. 9673-9680, (2018) (PubMed).
: "Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells." in: BioMed research international, Vol. 2016, pp. 1087647, (2017) (PubMed).
: "Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 48, pp. 15996-6011, (2015) (PubMed).
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: "Sesquiterpene lactone 6-O-angeloylplenolin reverses vincristine resistance by inhibiting YB-1 nuclear translocation in colon carcinoma cells." in: Oncology letters, Vol. 15, Issue 6, pp. 9673-9680, (2018) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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