ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
特异性
IgG (H&L)
产品特性
Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm
纯化方法
Preadsorption: Solid phase absorption
免疫原
Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
限制
仅限研究用
状态
Lyophilized
溶解方式
Restore with deionized water (or equivalent)
浓度
1.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液
Gentamicin sulfate
注意事项
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件
4 °C
Klaric, Thomas, Dottori, Leong, Koblar, Lewis: "A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells." in: Stem cell research & therapy, Vol. 5, Issue 3, pp. 64, (2015) (PubMed).
Gawriluk, Ko, Hong, Christenson, Rucker: "Beclin-1 deficiency in the murine ovary results in the reduction of progesterone production to promote preterm labor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 40, pp. E4194-203, (2015) (PubMed).
Tidjane, Hachem, Zaid, Merhi, Gaboury, Girolami, Couture: "A primary role for kinin B1 receptor in inflammation, organ damage, and lethal thrombosis in a rat model of septic shock in diabetes." in: European journal of inflammation, Vol. 13, Issue 1, pp. 40-52, (2015) (PubMed).