兔 anti-Pig IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
-
- 抗原 See all IgG products
- IgG
-
抗原表位
- Heavy & Light Chain
-
适用
- Pig
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- Alkaline Phosphatase (AP)
-
应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG (H&L)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: Solid phase absorption
- 过滤
- Sterile filtered
- 免疫原
-
Immunogen: Swine IgG whole molecule
- 亚型
- IgG
-
-
- 应用备注
-
Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Swine IgG antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:14,000
Western Blot Dilution: 1:500 - 1:2,500
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
-
Synonyms: Rabbit Anti-Swine IgG alkaline phosphatase Conjugated Antibody, Rabbit Anti-Swine IgG Antibody alk phos Conjugation
Background: Anti-Swine IgG (H&L) generated in rabbit detects swine Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Swine IgG is conjugated to alkaline phosphatase.
-