山羊 anti-Pig IgG (Heavy & Light Chain) Antibody (FITC)
Rockland 614-1202
WB, FACS, FLISA, FM, DB
Polyclonal
IgG
FITC
2 images
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- Pig
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)
- Supplier Product No.
- 614-1202
- Supplier
- Rockland
- 原理
- Swine IgG (H&L) Antibody Fluorescein Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Swine IgG and Swine Serum.
- 产品特性
- Anti-Swine IgG secondary antibody generated in goat detects specifically swine IgG. This secondary antibody anti-Swine is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads.
- 免疫原
- Optional[Immunogen]: Swine IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 2.33
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Goat anti-Pig IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
- Application Note: Anti-Swine IgG (H&L) Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Swine IgG Antibody is conjugated to Fluorescein.
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