山羊 anti-Pig IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- Pig
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG (H&L)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: immunoaffinity chromatography using Swine IgG coupled to agarose
- 免疫原
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Immunogen: Swine IgG whole molecule
- 亚型
- IgG
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Goat anti-Pig IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, LF Polyclonal IgG unconjugated
Rabbit anti-Pig IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal unconjugated
Goat anti-Pig IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Pig IgG (Heavy & Light Chain) antibody (HRP)ELISA Polyclonal IgG HRP
Rabbit anti-Pig IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Pig IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: This product has been assayed against 1.0 μg of Swine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:30,000 to 1:150,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Aliquot to Avoid repeated freezing and thawing.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat Anti Swine IgG Biotin Conjugated Antibody, Goat Anti-Swine IgG Biotin Conjugated Antibody, Goat Anti-Swine IgG Antibody Biotin Conjugation
Background: Anti-Swine IgG (H&L) generated in goat detects swine Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Swine IgG is conjugated to Biotin.
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