兔 anti-小鼠 IgG (Heavy & Light Chain) Antibody
(4 references)
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 610-4120
- Supplier
- Rockland
- 原理
- Mouse IgG (H&L) Antibody Pre-adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis (IEP) resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
- 产品特性
- Anti-Mouse IgG Antibody generated in rabbit detects reactivity to Mouse IgG gamma chain.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-Mouse IgG was produced by repeated immunization with mouse IgG whole molecule in rabbit
Immunogen Type: Native Protein
- 亚型
- IgG
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Goat anti-Mouse IgG antibody (DyLight 488) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 800)WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
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- 应用备注
- Application Note: Anti-Mouse IgG (H&L) Antibody has been tested by ELISA and is suitable for immunoblotting (Western or dot blot), ELISA, CUT&RUN, and immunohistochemistry. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:40,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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: "Preparation of optimized concanavalin A-conjugated Dynabeads® magnetic beads for CUT&Tag." in: PLoS ONE, Vol. 16, Issue 11, pp. e0259846, (2021) (PubMed).
: "H1 histones control the epigenetic landscape by local chromatin compaction. ..." in: Nature, (2020) (PubMed).
: "Mesenchymal Stromal/stem Cell-derived Extracellular Vesicles Promote Human Cartilage Regeneration In Vitro." in: Theranostics, Vol. 8, Issue 4, pp. 906-920, (2019) (PubMed).
: "Recombinant phosphatidylserine-binding nanobodies for targeting of extracellular vesicles to tumor cells: a plug-and-play approach." in: Nanoscale, Vol. 10, Issue 5, pp. 2413-2426, (2019) (PubMed).
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: "Preparation of optimized concanavalin A-conjugated Dynabeads® magnetic beads for CUT&Tag." in: PLoS ONE, Vol. 16, Issue 11, pp. e0259846, (2021) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This anti-Mouse secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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