兔 anti-小鼠 IgG (Heavy & Light Chain) Antibody (HRP)
(6 references)
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 610-4302
- Supplier
- Rockland
- 原理
- Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
- 产品特性
- Anti-Mouse IgG3 Fluorescein Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain).
- 纯化方法
- HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
- 免疫原
- Optional[Immunogen]: Mouse IgG whole molecule
- 亚型
- IgG
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Goat anti-Mouse IgG antibody (DyLight 488) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 800)WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
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- 应用备注
- Application Note: Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in rabbit detects specifically Mouse IgG whole molecule. Anti-Mouse IgG peroxidase antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:10,000 - 1:30,000 ELISA Dilution: 1:50,000 - 1:300,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store mouse secondary antibody conjugate at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells." in: Molecular medicine reports, Vol. 26, Issue 4, (2022) (PubMed).
: "Introduction of Two Prolines and Removal of the Polybasic Cleavage Site Lead to Higher Efficacy of a Recombinant Spike-Based SARS-CoV-2 Vaccine in the Mouse Model." in: mBio, Vol. 12, Issue 2, (2021) (PubMed).
: "An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening." in: Current protocols in microbiology, Vol. 58, Issue 1, pp. e108, (2020) (PubMed).
: "Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1." in: Frontiers in cell and developmental biology, Vol. 8, pp. 603688, (2020) (PubMed).
: "Transcutaneous Codelivery of Tumor Antigen and Resiquimod in Solid-in-Oil Nanodispersions Promotes Antitumor Immunity." in: ACS biomaterials science & engineering, Vol. 5, Issue 5, pp. 2297-2306, (2019) (PubMed).
: "The influenza virus hemagglutinin head evolves faster than the stalk domain." in: Scientific reports, Vol. 8, Issue 1, pp. 10432, (2018) (PubMed).
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: "Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells." in: Molecular medicine reports, Vol. 26, Issue 4, (2022) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated. Rockland produces highly active antibodies and conjugates against mouse immunoglobulins. Anti-Mouse Secondary Antibodies are affinity-purified polyclonal antibodies with well-characterized specificity for mouse immunoglobulin classes, subclasses, and fragments. They are available as unlabeled antibodies as well as conjugates of alkaline phosphatase (AP), horseradish peroxidase (HRP), fluorescent conjugates, and biotin. This Anti-MOUSE IgG generated in rabbit is designed to detect heavy and light chains (H&L) and has been Peroxidase (HRP) conjugated. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays. These secondary antibodies are used throughout various types of assays, including ELISA or Western Blot, Immunohistochemistry, Flow Cytometry. Optimal secondary antibody requires knowledge of the detection assay.
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