山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Immunoprecipitation (IP), Lateral Flow (LF)
- 特异性
- IgG (H&L)
- 交叉反应
- 小鼠
- 产品特性
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Anti-Mouse IgG whole molecule antibody generated in goat detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm - 纯化方法
- Preadsorption: Solid phase absorption
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
Immunogen Type: Native Protein
- 亚型
- IgG
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Goat anti-Mouse IgG antibody (DyLight 488) - Preadsorbed
WB, FLISA, FM Polyclonal IgG DyLight 488
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Rabbit anti-Mouse IgG antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Atto 594) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 594
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Atto 425) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 425
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 647N
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Goat anti-Mouse IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Mouse IgG affinity purified secondary antibody is generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse IgG secondary antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Immunoprecipitation Dilution: Yes
FLISA Dilution: Yes
ELISA Dilution: 1:25,000
Flow Cytometry Dilution: Yes
Western Blot Dilution: 1:3,000 - 1:15,000
IF Microscopy Dilution: Yes
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat anti-Mouse IgG Secondary Antibody, Mouse Secondary Antibody, GAM Antibody, Gt-a-Ms antibody, anti-mouse secondaries
Background: Anti-Mouse IgG Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This unconjugated secondary antibody has been validated and optimized yielding good sensitivity and reproducible results with Rockland's primary antibodies.
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