ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
特异性
IgG (H&L)
产品特性
Anti-Mouse IgG whole molecule antibody generated in donkey detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm
免疫原
Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other peroxidase antibody-based enzymatic assays requiring lot-to-lot consistency.
限制
仅限研究用
状态
Lyophilized
溶解方式
Restore with deionized water (or equivalent)
浓度
1.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液
Gentamicin sulfate
注意事项
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件
4 °C
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Schorch, Heni, Zahaf, Brummer, Mione, Schmidt, Papatheodorou, Aktories: "Targeting oncogenic Ras by the Clostridium perfringens toxin TpeL." in: Oncotarget, Vol. 9, Issue 23, pp. 16489-16500, (2018) (PubMed).
Klaric, Thomas, Dottori, Leong, Koblar, Lewis: "A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells." in: Stem cell research & therapy, Vol. 5, Issue 3, pp. 64, (2015) (PubMed).
Hemmasi, Czulkies, Schorch, Veit, Aktories, Papatheodorou: "Interaction of the Clostridium difficile Binary Toxin CDT and Its Host Cell Receptor, Lipolysis-stimulated Lipoprotein Receptor (LSR)." in: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14031-44, (2015) (PubMed).
Litjens, Nguyen, Castro, Aromataris, Jones, Barritt, Rychkov: "Phospholipase C-gamma1 is required for the activation of store-operated Ca2+ channels in liver cells." in: The Biochemical journal, Vol. 405, Issue 2, pp. 269-76, (2007) (PubMed).
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