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- 抗原 See all IgG products
- IgG
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抗原表位
- Fc Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- FLISA, Fluorescence Microscopy (FM)
- 特异性
- IgG F(c)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Human IgG F(c) fragment
- 亚型
- IgG
- Labeling Ratio
- 2.6
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Goat anti-Human IgG (Fc Region) antibody (PE) - Preadsorbed
FACS, FM Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgG (Fc Region) antibody (Alkaline Phosphatase (AP))ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Human IgG (Fd Region) antibody (FITC)ELISA, FLISA Polyclonal IgG FITC
Goat anti-Human IgG antibody (HRP) - PreadsorbedELISA, FACS, FLISA Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Human IgG (Fd Region) antibody (HRP)ELISA, FLISA Polyclonal IgG HRP
Goat anti-Human IgG (Fd Region) antibody (Alkaline Phosphatase (AP))ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Human IgG (Fc Region) antibody (PE) - PreadsorbedELISA, FLISA Polyclonal IgG PE
Goat anti-Human IgG (Fc Region) antibody (Biotin)ELISA, FLISA Polyclonal IgG Biotin
Goat anti-Human IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
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- 应用备注
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Application Note: Anti-Human IgG F(c) Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 说明
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Excitation/Emission wavelength: 494 nm/514 nm
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: rabbit Anti-Human IgG F(c) Antibody fluorescein Conjugated, rabbit Anti-Human IgG Fc fragment Antibody fluorescein Conjugated, rabbit Anti-Human IgG Fc Antibody FITC Conjugated
Background: Anti-Human IgG F(c) Fluorescein Conjugated generated in rabbit detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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