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山羊 anti-人 IgG (Fc Region) Antibody (FITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG FITC
产品编号 ABIN101661
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2382
    • 510
    • 424
    • 207
    • 131
    • 129
    • 95
    • 29
    • 28
    • 28
    • 22
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Fc Region
    适用
    • 722
    • 699
    • 602
    • 477
    • 403
    • 243
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    • 176
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    • 138
    • 113
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    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
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    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
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    • 241
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    • 111
    • 33
    • 31
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    • 15
    • 11
    • 11
    • 1
    山羊
    克隆类型
    • 3433
    • 175
    • 1
    多克隆
    标记
    • 588
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    • 416
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    FITC
    应用范围
    • 1953
    • 1836
    • 1807
    • 1520
    • 933
    • 909
    • 652
    • 478
    • 380
    • 236
    • 236
    • 234
    • 106
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    • 86
    • 67
    • 53
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    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    特异性
    IgG (H&L)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Human IgG F(c) fragment

    亚型
    IgG
    Labeling Ratio
    3.7
  • 应用备注

    Application Note: Anti-Human IgG F(c) Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    说明

    This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. nm
    Excitation/Emission wavelength: 494 nm/514 nm

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: goat anti-Human IgG F(c) fluorescein Conjugated Antibody, goat anti-Human IgG F(c) fragment antibody FITC conjugation

    Background: Anti-Human IgG F(c) Fluorescein Conjugated generated in goat detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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