兔 anti-人 IgG (F(ab')2 Region) Antibody
(1 reference)
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- 抗原 See all IgG products
- IgG
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抗原表位
- F(ab')2 Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
- Supplier Product No.
- 609-4118
- Supplier
- Rockland
- 原理
- Human IgG F(ab')2 Antibody pre-absorbed
- 交叉反应 （详细）
- Minimal crossreactivity against MOUSE Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.
- 产品特性
- Anti-Human IgG (H&L) generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.
- 纯化方法
- Anti-Human IgG F(ab')2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
- Optional[Immunogen]: Anti-Human IgG F(ab')2 fragment was produced in rabbit by repeated immunization with human IgG F(ab')2 fragment.
- 亚型
- IgG
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Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgG (Fc Region) antibody (Biotin)ELISA, FLISA Polyclonal IgG Biotin
Goat anti-Human IgG (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
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- 应用备注
- Application Note: Anti-Human IgG F(ab')2 antibody has been tested by ELISA and dot blot and is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:3,000 - 1:15,000 ELISA Dilution: 1:350,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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: "Efficacy of a recombinant single-chain fragment variable region, VasSF, as a new drug for vasculitis." in: Drug design, development and therapy, Vol. 13, pp. 555-568, (2019) (PubMed).
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: "Efficacy of a recombinant single-chain fragment variable region, VasSF, as a new drug for vasculitis." in: Drug design, development and therapy, Vol. 13, pp. 555-568, (2019) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Anti-Human IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG F(ab')2 Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
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