山羊 anti-豚鼠 IgG (Fc Region) Antibody (Biotin) - Preadsorbed
-
- 抗原 See all IgG products
- IgG
-
抗原表位
- Fc Region
-
适用
- 豚鼠
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- Biotin
-
应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG F(c)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
-
Immunogen: Guinea Pig IgG F(c) fragment
- 亚型
- IgG
-
Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Donkey anti-Guinea Pig IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, WB Polyclonal IgG unconjugated
Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, WB Polyclonal IgG HRP
Rabbit anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP)ELISA Polyclonal IgG HRP
Donkey anti-Guinea Pig IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Guinea Pig IgG (Fc Region) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
-
- 应用备注
-
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: This product has been assayed against 1.0 μg of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:35,000 to 1:150,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 2.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Aliquot to Avoid repeated freezing and thawing.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
-
Synonyms: Goat Anti-Guinea Pig IgG F(c) Antibody Biotin Conjugated, Goat Anti Guinea Pig IgG Fc Fragment Antibody Biotin Conjugated, Goat Anti Guinea Pig IgG Fc Antibody Biotin Conjugated
Background: Anti-Guinea Pig IgG F(c) Biotin generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of Guinea Pig IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
-
