兔 anti-豚鼠 IgG (F(ab')2 Region) Antibody
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- 抗原 See all IgG products
- IgG
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抗原表位
- F(ab')2 Region
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适用
- 豚鼠
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG F(ab')2
- 产品特性
- Concentration Definition: by Refractometry
- 纯化方法
- This product was prepared from monospecific antiserum by delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Guinea Pig IgG F(ab')2. No reaction was observed against Guinea Pig IgG F(c).
- 免疫原
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Immunogen: Guinea Pig IgG F(ab')2 fragment
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 90 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: rabbit anti-Guinea Pig IgG F(ab')2 fragment Antibody, rabbit anti-Guinea Pig IgG Fab2 fragment Antibody, rabbit anti Guinea Pig IgG Fab2
Background: Anti-Guinea Pig IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Guinea Pig IgG F(c) will not bind Guinea Pig IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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