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山羊 anti-豚鼠 IgG (F(ab')2 Region) Antibody

ELISA, IHC, WB Polyclonal unconjugated
产品编号 ABIN101304
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
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    F(ab')2 Region
    适用
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    豚鼠
    宿主
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    山羊
    克隆类型
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    多克隆
    应用范围
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG F(ab')2
    产品特性
    Concentration Definition: by Refractometry
    纯化方法
    This product was prepared from monospecific antiserum by delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, guinea pig IgG, guinea pig serum, Guinea Pig IgG F(ab')2.  No reaction was observed against Guinea Pig IgG F(c).
    免疫原

    Immunogen: Guinea Pig IgG F(ab')2 fragment

  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    90 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: goat anti-Guinea Pig IgG F(ab')2 fragment Antibody, goat anti-Guinea Pig IgG Fab2 fragment Antibody, goat anti Guinea Pig IgG Fab2

    Background: Anti-Guinea Pig IgG F(ab')2 Antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Guinea Pig IgG F(c) will not bind Guinea Pig IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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