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兔 anti-豚鼠 IgG (Heavy & Light Chain) Antibody

ELISA, IHC, WB Polyclonal unconjugated
产品编号 ABIN101287
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
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    Heavy & Light Chain
    适用
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    豚鼠
    宿主
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    克隆类型
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    多克隆
    应用范围
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG (H&L)
    产品特性
    Concentration Definition: by Refractometry
    纯化方法
    This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Guinea Pig IgG and Guinea Pig Serum.
    免疫原

    Immunogen: Guinea Pig IgG whole molecule

  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    70 mg/mL
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: rabbit anti-Guinea Pig IgG Antibody, rabbit anti Guinea Pig IgG

    Background: Anti-Guinea Pig IgG Antibody generated in rabbit detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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