Anti-Goat IgG F(c) peroxidase conjugated antibody generated in rabbit detects specifically goat IgG F(c). This secondary peroxidase conjugated antibody anti-Goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm
免疫原
Anti-Goat IgG was produced by repeated immunization with Goat IgG fragment in rabbit.
Goat IgG F(c) peroxidase conjugated antibody has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product. Anti-Goat IgG F(c) peroxidase conjugated antibody has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product.
限制
仅限研究用
状态
Lyophilized
溶解方式
Restore with deionized water (or equivalent)
浓度
2.0 mg/mL
缓冲液
0.01 M Potassium Phosphate, 0.14 M Sodium Chloride, pH 7.4 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA), IgG and Protease free Preservative: 0.01 % (w/v) Gentamicin Sulfate
储存液
Gentamicin sulfate
注意事项
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件
4 °C
Ma, Yan, Huang, Zhao, Zhu: "Downregulation of human epidermal growth factor receptor 2 by short hairpin RNA increases chemosensitivity of human ovarian cancer cells." in: Oncology letters, Vol. 9, Issue 5, pp. 2211-2217, (2015) (PubMed).