山羊 anti-驴 IgG (Heavy & Light Chain) Antibody - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 驴
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG (H&L)
- 交叉反应
- 驴
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: immunoaffinity chromatography using Donkey IgG coupled to agarose beads
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Donkey IgG whole molecule
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Donkey IgG antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: goat anti-Donkey IgG Antibody
Background: Anti-Donkey IgG Antibody generated in goat detects donkey IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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