山羊 anti-犬 IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 犬
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG (H&L)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: immunoaffinity chromatography using Dog IgG coupled to agarose beads
- 免疫原
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Immunogen: Dog IgG whole molecule
- 亚型
- IgG
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Rabbit anti-Dog IgG (Fc Region) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated
Rabbit anti-Dog IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin F(ab')2 fragment
Rabbit anti-Dog IgG (Fc Region) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: This product has been assayed against 1.0 μg of Dog IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 20,000 to 1:60,000 is suggested for this product.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Aliquot to Avoid repeated freezing and thawing.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: goat anti Dog IgG Antibody Biotin Conjugated, goat anti-Dog IgG Antibody Biotin Conjugated
Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Dog IgG (H&L) antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
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