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兔 anti-小鸡 IgG (F(ab')2 Region) Antibody (FITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG FITC
产品编号 ABIN101063
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2333
    • 513
    • 418
    • 206
    • 132
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    • 98
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    • 1
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    • 1
    F(ab')2 Region
    适用
    • 728
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    小鸡
    宿主
    • 1762
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    克隆类型
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    多克隆
    标记
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    FITC
    应用范围
    • 1960
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    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    特异性
    IgG F(ab')2
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Chicken IgG F(ab')2 fragment

    亚型
    IgG
    Labeling Ratio
    3.2
  • 应用备注

    Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    说明

    Excitation/Emission wavelength: 494 nm/514 nm

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.5 mg/mL
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: rabbit anti-Chicken IgG F(ab')2 Antibody Fluorescein Conjugation, rabbit anti-Chicken IgG Fab2 Fragment FITC Conjugated Antibody

    Background: Anti-Chicken IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Chicken IgG F(c) will not bind Chicken IgG F(ab')2 Molecules.

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