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兔 anti-小鸡 IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP))

Rockland 603-4504 ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Rockland
产品编号 ABIN101060
Supplier Product No.: 603-4504
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2403
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    • 129
    • 128
    • 67
    • 29
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    • 28
    • 21
    • 9
    • 5
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    • 1
    • 1
    F(ab')2 Region
    适用
    • 720
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    • 1
    小鸡
    宿主
    • 1761
    • 1548
    • 569
    • 238
    • 112
    • 111
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    • 21
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    • 15
    • 11
    • 11
    克隆类型
    • 3389
    • 175
    • 1
    多克隆
    标记
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    Alkaline Phosphatase (AP)
    应用范围
    • 2032
    • 1981
    • 1835
    • 1784
    • 983
    • 920
    • 896
    • 486
    • 419
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    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
    Supplier Product No.
    603-4504
    Supplier
    Rockland
    原理
    Chicken IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab')2 and Chicken Serum.  No reaction was observed against Chicken IgG F(c).
    产品特性
    Anti-Chicken IgG F(c) Antibody detects specifically Chicken IgG F(c). It is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
    纯化方法
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    过滤
    Sterile filtered
    免疫原
    Optional[Immunogen]: Chicken IgG F(ab')2 fragment
    亚型
    IgG
  • 应用备注
    Application Note: Anti-Chicken IgG F(ab')2 Alkaline Phosphatase antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:3,500 Other: User Optimized
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    Anti-Chicken IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Chicken IgG F(c) will not bind Chicken IgG F(ab')2 Molecules.
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