兔 anti-小鸡 IgY (Heavy & Light Chain) Antibody (Biotin)
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- 抗原 See all IgY products
- IgY
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抗原表位
- Heavy & Light Chain
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适用
- 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 603-4602
- Supplier
- Rockland
- 原理
- Chicken IgG (H&L) Secondary Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Chicken IgG / IgY. Some light chain cross reactivity may be observed against chicken immunoglobulins.
- 产品特性
- Anti-Chicken IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Chicken IgG F(c) will not bind Chicken IgG F(ab')2 Molecules.
- 纯化方法
- Biotin Conjugated Anti-Chicken IgG / IgY secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG / IgY coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
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Immunogen: Anti-Chicken IgY / IgG whole molecule was produced by repeated immunization with Chicken IgY / IgG whole molecule in rabbit.
Immunogen Type: Native Protein
- 亚型
- IgG
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Chicken anti-Chicken IgY antibody (DyLight 633)
IF, WB Polyclonal IgY DyLight 633
Chicken anti-Chicken IgY antibody (DyLight 405)IF, WB Polyclonal IgY DyLight 405
Chicken anti-Chicken IgY antibody (DyLight 488)IF, WB Polyclonal IgY DyLight 488
Goat anti-Chicken IgY (Heavy & Light Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Chicken anti-Chicken IgY antibody (DyLight 550)IF, WB Polyclonal IgY DyLight 550
Mouse anti-Chicken IgY antibody (PE)ELISA Monoclonal G-1 IgG1 PE
Goat anti-Chicken IgY (Heavy & Light Chain) antibody (PE)ELISA Polyclonal IgG PE
Mouse anti-Chicken IgY antibodyELISA Monoclonal G-1 IgG1 unconjugated
Mouse anti-Chicken IgY antibody (Biotin)ELISA Monoclonal G-1 IgG1 Biotin
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- 应用备注
- Application Note: Secondary antibodies are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:500,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 1.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgY
- 别名
- IgY,IgG (IgY 产品)
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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